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IOP Publishing, Journal of Micromechanics and Microengineering, 5(20), p. 055010, 2010

DOI: 10.1088/0960-1317/20/5/055010

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Injection molded chips with integrated conducting polymer electrodes for electroporation of cells

This paper is available in a repository.
This paper is available in a repository.

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Abstract

We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, ±50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.