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Public Library of Science, PLoS ONE, 4(10), p. e0118803, 2015

DOI: 10.1371/journal.pone.0118803

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Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

Journal article published in 2015 by Armon Reza Sharei, Radiana Trifonova, Siddharth Jhunjhunwala, George C. Hartoularos, Alexandra T. Eyerman, Abigail Lytton-Jean, Mathieu Angin, Siddhartha Sharma, Roberta Poceviciute, Shirley Mao, Megan Heimann, Sophia Liu, Tanya Talkar, Omar F. Khan, Marylyn Addo and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.