The ability to transmit signals with high information spectral density (ISD) using low-complexity and cost-effective transceivers is essential for short- and medium-haul optical communication systems. Consequently, spectrally efficient direct detection transceiver-based solutions are attractive for such applications. In this paper, we experimentally demonstrate the wavelength-division multiplexed (WDM) transmission of 7× 12 GHz-spaced dispersion pre-compensated Nyquist pulse-shaped 16-QAM subcarrier modulated channels operating at a net bit rate of 24 Gb/s per channel, and achieving a net optical ISD of 2.0 b/s/Hz. The direct detection receiver used in our experiment consisted of a single-ended photodiode and a single analog-to-digital converter. The carrier-to-signal power ratio at different values of optical signal-to-noise ratio was optimized to maximize the receiver sensitivity performance. The transmission experiments were carried out using a recirculating fiber loop with uncompensated standard single-mode fiber and EDFA-only amplification. The maximum achieved transmission distances for single channel and WDM signals were 727 and 323 km below the bit-error ratio of {3.8\times 10^{-3}}, respectively. To the best of our knowledge, this is the highest achieved ISD for WDM transmission in direct detection links over such distances.