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Elsevier, Cell, 7(163), p. 1692-1701, 2015

DOI: 10.1016/j.cell.2015.11.029



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Structural Basis of Vesicle Formation at the Inner Nuclear Membrane

This paper is available in a repository.
This paper is available in a repository.

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Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear en- velope. Cargo is recruited, enveloped at the inner nu- clear membrane (INM), and delivered by membrane fusion at the outer nuclear membrane. To understand the structural underpinning for this trafficking, we investigated nuclear egress of progeny herpesvirus capsids where capsid envelopment is mediated by two viral proteins, forming the nuclear egress com- plex (NEC). Using a multi-modal imaging approach, we visualized the NEC in situ forming coated vesicles of defined size. Cellular electron cryo-tomography revealed a protein layer showing two distinct hexag- onal lattices at its membrane-proximal and mem- brane-distant faces, respectively. NEC coat architec- ture was determined by combining this information with integrative modeling using small-angle X-ray scattering data. The molecular arrangement of the NEC establishes the basic mechanism for budding and scission of tailored vesicles at the INM.