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Springer, Biomolecular NMR Assignments, 1(10), p. 13-23, 2015

DOI: 10.1007/s12104-015-9629-8

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Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase

Journal article published in 2015 by Thomas Wiegand, Carole Gardiennet, Francesco Ravotti, Alexandre Bazin, Britta Kunert, Denis Lacabanne ORCID, Riccardo Cadalbert, Peter Güntert, Laurent Terradot, Anja Böckmann, Beat H. Meier
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We present solid-state NMR assignments of the N-terminal domain of the DnaB helicase from Helicobacter pylori (153 residues) in its microcrystalline form. We use a sequential resonance assignment strategy based on three-dimensional NMR experiments. The resonance assignments obtained are compared with automated resonance assignments computed with the ssFLYA algorithm. An analysis of the (13)C secondary chemical shifts determines the position of the secondary structure elements in this α-helical protein.