Gamma interferon (IFN-gamma) production has been attributed a protective role against abortion in Neospora-infected dairy cows. This study investigate possible relationships between plasma levels of the N. caninum-specific immunoglobulin (Ig) isotypes IgG1 and IgG2 and IFN-gamma production throughout gestation in dairy cows naturally infected with the parasite. Data were obtained from 31 pregnant Neospora-seropositive animals. Blood samples were collected on gestation days 40, 90, 120, 150, 180 and 210 or until abortion. Ten pregnancies ended in abortion. One of the 11 cows producing IFN-gamma suffered abortion (9.1% of the cows producing IFN-gamma). Non-aborting cows producing IFN-gamma (n=10) showed a significantly higher IgG2/IgG1 antibody ratio throughout the gestation period than cows not producing IFN-gamma (n=11). A significant negative effect of IFN-gamma production on IgG1 antibodies was also observed, while IFN-gamma production did not affect IgG2 antibody levels. In contrast, higher levels of IgG2 antibodies compared to IgG1 antibodies were observed over the whole of gestation in aborting animals, both in those not producing IFN-gamma and in the single aborting cow that produced the cytokine. Our findings indicate that a Th1 immune response, in which IgG2 antibodies prevail, could be protective against N. caninum abortion, but only in the presence of IFN-gamma production. On their own, elevated IgG2 antibody titres appear to be insufficient to protect dams chronically infected with N. caninum against abortion.