Published in
Wiley, Microscopy Research and Technique, 1(63), p. 12-17, 2003
DOI: 10.1002/jemt.10425
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Notes on Theory and Experimental Conditions behind Two-Photon Excitation Microscopy
Journal article published in 2003 by
Alessandro Esposito,
Federico Federici,
Cesare Usai,
Fabio Cannone,
Giuseppe Chirico,
Maddalena Collini 
,
Alberto Diaspro
,
Alberto Diaspro
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Abstract
This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission.