Published in
Elsevier, Forensic Science International: Genetics Supplement Series, 1(4), p. e119-e120
DOI: 10.1016/j.fsigss.2013.10.061
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Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis
Journal article published in 2013 by
Dmitriy A. Khodakov,
Anastasia S. Khodakova,
Adrian Linacre,
Amanda V. Ellis 
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Abstract
The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.