In this study, we developed five microsatellite loci from an enriched genomic library con- structed for the pygmy treeshrew ( Tupaia minor ), and adapted another two from a previous study on the common treeshrew ( Tupaia glis ), for use in studying mating and dispersal patterns in Bornean treeshrews. We screened 32 plain treeshrew ( Tupaia longipes ) and 54 large treeshrew ( Tupaia tana ) individuals at these loci. Polymorphism ranged from two to 13 alleles, and heterozygosity ranged from 0.29 to 0.88. These results indicate the general utility of these microsatellites for genetic analyses in other Tupaia spp.