7 Corresponding author 8 Retired and associated as voluntary researcher Abstract A check-list containing 196 species of reef fishes recorded at the Laje de Santos Marine State Park is presented. Most of them occur along the tropical western Atlantic or occur on both sides of the Atlantic Ocean. A minor part ranges to the temperate rocky reefs of Patagonia or are endemics to southeastern Brazil. Moringua edwardsi, Antennarius multiocella- tus, Scorpaena dispar, Aulostomus strigosus, Lutjanus buccanella, Mulloidichthys martinicus and Halichoeres penrosei have here their ranges extended southwards to the São Paulo coast. Basic data on species abundance at the study site, dis- tribution of species between habitat types, trophic structure, feeding symbiotic associations, and conservation are com- mented upon.