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American Association for the Advancement of Science, Science, 6094(337), p. 591-595, 2012

DOI: 10.1126/science.1218716

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Opening and Closing of the Bacterial RNA Polymerase Clamp

Journal article published in 2012 by Anirban Chakraborty, Dongye Wang, Yon W. Ebright, You Korlann, Ekaterine Kortkhonjia, Taiho Kim, Saikat Chowdhury ORCID, Sivaramesh Wigneshweraraj, Herbert Irschik, Rolf Jansen, B. Tracy Nixon, Jennifer Knight, Shimon Weiss, Richard H. Ebright
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Clamping Down Crystal structures of RNA polymerase show that a “clamp” region which surrounds the DNA binding site can adopt conformations ranging from a closed to an open state. Chakraborty et al. (p. 591 ) used single-molecule fluorescence energy transfer experiments to detect the clamp's conformational changes in solution during the transcription cycle. The results support a model in which a clamp opening allows DNA to be loaded into the active-center cleft and unwound. Direct interactions with DNA likely trigger clamp closure upon formation of a catalytically competent transcription initiation complex.