The increased uptake and storage of lipids in the liver is an important feature of steatotic liver diseases. The fatty acid translocase/scavenger receptor CD36 facilitates the hepatic uptake of lipids. We investigated if RRR-α-tocopherol (αT) alone or in combination with atorvastatin (ATV) is capable of preventing hepatic lipid accumulation via down-regulation of CD36. To this end, Dunkin-Hartley guinea pigs were fed a control (5% fat) or a high-fat control diet (21% fat, 0.15% cholesterol) or the high-fat control diet fortified with αT (250 mg/kg diet), ATV (300 mg/kg diet) or both ATV + αT for six weeks. Hepatic triacylglyerols, hepatic protein and mRNA expression of CD36 as well as the mRNA expression of the controlling nuclear receptors LXRα, PXR and PPARγ were determined. Animals fed the high-fat control diet accumulated significantly more triacylglycerols in the liver than control animals. This was significantly reduced by ATV and numerically by αT and ATV + αT. Hepatic CD36 protein concentrations were significantly higher in the high-fat than in the control group, and both αT and ATV reduced CD36 expression to the level observed in the control group. However, no synergistic effect of the combined treatment was observed. Neither CD36 mRNA nor that of the nuclear receptors (LXRα, PXR and PPARγ) differed between groups, suggesting a post-translational regulatory mechanism. Our results indicate that orally administered atorvastatin and αT individually, but not synergistically, prevent diet-induced lipid-accumulation in the liver of guinea pigs by down-regulation of hepatic CD36 protein.