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American Society for Microbiology, Molecular and Cellular Biology, 10(7), p. 3842-3845, 1987

DOI: 10.1128/mcb.7.10.3842-3845.1987

American Society for Microbiology, Molecular and Cellular Biology, 10(7), p. 3842-3845, 1987

DOI: 10.1128/mcb.7.10.3842

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Posttranslational translocation of influenza virus hemagglutinin across microsomal membranes.

Journal article published in 1987 by Chuck C.-K. Chao, Phillip Ian Bird ORCID, Mary-Jane Gething, Joe Sambrook
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The biosynthesis of influenza virus hemagglutinin (HA) and its translocation across microsomal membranes were studied in a mammalian cell-free system. All forms of HA could be cotranslationally translocated with high efficiency. However, only truncated forms of HA were translocated after protein synthesis has been terminated. The efficiency of this posttranslational translocation was dependent on the extent of the truncation. Posttranslational translocation was ribosome dependent and occurred only in the presence of a functional N-terminal signal sequence. The molecular mechanism of protein targeting and translocation across the membrane of the endoplasmic reticulum is discussed.