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Taylor and Francis Group, Transcription, 3(1), p. 159-164
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- [www.ncbi.nlm.nih.gov] | PDF
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- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
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Serious complications in gene-expression studies with stress perturbation
Journal article published in 2010 by
Oskar Bruning,
Xiaolian Yuan,
Wendy Rodenburg,
Wendy Bruins,
Conny T. van Oostrom,
Han Rauwerda 
,
Floyd R. A. Wittink,
Martijs J. Jonker,
Annemieke de Vries,
Timo M. Breit
,
Floyd R. A. Wittink,
Martijs J. Jonker,
Annemieke de Vries,
Timo M. Breit
This paper is available in a repository.
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Abstract
Reanalysis of our UV study of p53-mutant mouse embryonic fibroblasts revealed an intriguing orchestration of massive transcriptome responses. However, close scrutiny of the data uncovered an affected mRNA/rRNA ratio, effectively inhibiting valid data analysis. UV-dose range-finding showed low-dose UV specific- and high-dose stress-related responses, which represent a plea for UV dose range-finding in experimental design.