New acrylic bone cements were prepared by incorporating silver oxide as potential antimicrobial agent in a polymethyl metacrylate (PMMA) matrix. In order to improve the biomineralisation and biocompatibitity of these cements, electrolytic deposition of collagen was performed in a three-electrode electrochemistry system. ATR FTIR and FT Raman analyses demonstrated that collagen adsorption depends on the silver content in samples. In vitro tests, performed in simulated body fluid (SBF) during three weeks of SBF incubation, revealed the release of Ag + by a mechanism involving Ca 2+ and Na + ions exchange. The mineralization process upon incubation was confirmed by the ATR FTIR spectra, which evidenced the phosphate characteristic bands of hydroxyapatite, depending on the silver oxide content in the samples.