Antibacterial activity studies of new molecules, either alone or in combination with existing antibiotics, are of great importance considering the resistance acquired by microorganisms in recent times. Linalool is a phyto-constituent found in the essential oils of various plant species. It is a monoterpene widely used in perfumery, cosmetics, and the food industries. Our objective was to determine the pharmacological effects produced on the bacterial strains Staphylococcus aureus, and Pseudomonas aeruginosa when combining standard antibiotics with linalool. The Minimum Inhibitory Concentration (MIC) was calculated using microdilution technique, where the linalool concentrations varied from 2 to 1024 μg /mL. Combinations with standard antibiotics were analyzed by the checkerboard method where the fractional inhibitory concentration (FIC) indices were calculated. Linalool, Imipenem, and Ciprofloxacin showed respective MIC antibacterial activities against S. aureus of 1024, 4, and 2 μg/mL. In S. aureus, the linalool with Imipenem association showed a synergistic effect (FIC = 0.0625); while with ciprofloxacin, the linalool showed additivity (FIC = 0.75). In P. aeruginosa, the Imipenem/linalool association was synergistic for both the ATCC and clinical strains (FIC = 0.0625). The association of linalool with ciprofloxacin was indifferent. We conclude that Linalool associated with existing standard antibiotics may increase antibacterial effectiveness, resulting in synergistic activity against bacterial strains of clinical importance. This makes the molecule potentially important for production of new, therapeutically effective drugs against resistant microorganisms. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.