A novel actinomycete, strain DS3030^T, was isolated from a deep sediment sample, collected from the southern Black Sea coast, Turkey, and was examined using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain DS3030^T was shown to belong to the genus Saccharomonospora and to be related most closely to Saccharomonospora marina XMU15^T (99.6 % similarity). Sequence similarities with other strains of the genus Saccharomonospora were lower than 97.0 %. The organism had chemical and morphological features typical of the genus Saccharomonospora . The cell wall of the novel strain contained meso-diaminopimelic acid, arabinose and galactose as diagnostic sugars. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinone was MK-9(H₄). Major fatty acids were iso-C_{16 : 0}, iso-C_{16 : 0} 2-OH and C_{16 : 1} cis 9. Phenotypic data clearly distinguished the new isolate from its closest relative, S. marina XMU15^T. The combined genotypic and phenotypic data and low DNA–DNA relatedness with its closest related strain reveal that strain DS3030^T represents a novel species of the genus, for which the name Saccharomonospora amisosensis sp. nov. is proposed. The type strain is DS3030^T ( = DSM 45685^T = KCTC 29069^T = NRRL B-24885^T).