Real-time PCR is the most sensitive method for detection and precise quantification of specific DNA sequences, but it is not usually applied as quantitative method in seafood. In general, benchmark techniques, mainly cycle-threshold (Ct), are the routine way for quantitative estimations, but they are not the most precise approach for a standard assay. In the present work, amplification data from European hake (Merluccius merluccius) DNA samples were accurately modelled by three sigmoid reparameterised equations where the lag phase parameter (lambda-c) from Richards equation with four parameters demonstrated to be the perfect substitute of Ct for PCR quantification. The concentration of primers and probes were subsequently optimized by means of that selected kinetic parameter. Finally, the linear correlation among DNA concentration and lambda-c was also confirmed.