Forward numerical models are used to investigate the effect of deformation regime on the development of olivine lattice-preferred orientations (LPO) and associated seismic anisotropy within continental deformation zones. LPO predicted to form by pure shear, simple shear, transpression, or transtension are compared to a database comprising ca. 200 olivine LPO from naturally deformed upper mantle rocks. This comparison suggests that simple shear or plane combinations of simple and pure shear are probably the dominant deformation regimes in the upper mantle. Seismic properties, calculated using the modeled olivine LPO, suggest that seismic anisotropy data may carry information on the deformation regimes active in the lithospheric mantle, although not all deformation regimes are characterized by a distinct seismic anisotropy signal. Transtensional deformation in continental rift systems should result in fast S-wave polarization and P-wave propagation directions oblique to the rift trend within the extended lithospheric mantle. Simple shear (wrench) or transpression in vertical deformation zones and pure shear (horizontal extension) result in similar seismic anisotropy. Simple shear or widening–thinning shear may, however, induce obliquity between seismic and magnetotelluric electrical conductivity anisotropy data. Similarly, it is not possible to distinguish between simple shear or lengthening–thinning shear (plane transpression) in horizontal deformation zones (thrusts) and pure shear (vertical contraction/horizontal extension). In all cases, the polarization direction of the fast split S-wave and the fast P-wave direction parallels the flow direction, but the anisotropy for both Pn- and S-waves is lower in horizontal structures than in vertical ones. Finally, several deformations show an isotropic response to SKS and/or Pn waves, suggesting that seismic isotropy does not necessarily imply absence (or heterogeneity) of deformation. There is a good agreement between model predictions and seismic anisotropy data in both transtensional and transpressional zones, suggesting coupled deformation of the crust and mantle. Oblique fast S-wave polarization directions in the East African rift, for instance, may result from an early transtensional deformation in the mantle lithosphere below the rift system. In contrast, most thrust belts display fast S-waves polarized parallel to the trend of the belt. One possible interpretation is that the upper mantle is decoupled from the crust in these areas.