This study points to changes in the properties of liposomes and its functional qualities in task of release of encapsulated drug doxorubicin according to the content of cholesterol in the phospholipid bilayer. The influence of sodium dodecyl sulphate (SDS) addition to liposomal variants was also evaluated too. Three variants of liposomes differing in various concentrations of cholesterol were assessed. Firstly, we focused on the toxicity of all liposomal variants to Staphyloccocus aureus and it was found that the content of cholesterol increases the IC50 values of encapsulated doxorubicin in liposome with higher concentration of cholesterol than in liposome without cholesterol even four times. Further, the new approach to comparing the influence of various liposomes on detection of encapsulated doxorubicin using the electrochemical detection with construction of differential hydrodynamic voltammograms was used. Finally, the fluorescence detection was used to confirm the release of encapsulated doxorubicin from liposomes after the addition of detergent SDS. In this work we demonstrated the suitability of number of methods including electrochemistry for studying of effect of substances such substance as SDS and cholesterol on the lipid functional qualities.