Varicella-zoster virus (VZV) is a member of the Herpesviridae family, which causes varicella (chicken pox) and herpes zoster (shingles) in humans. Here, we report the complete genome sequence of varicella-zoster virus, isolated from a vesicular fluid sample, revealing the circulation of VZV clade VIII in Mexico. FOOTNOTES Address correspondence to Fabiola Garcés-Ayala, garces.fabiola23{at}gmail.com, or José Ernesto Ramirez-González, ernesto.ramirez{at}salud.gob.mx. Citation Garcés-Ayala F, Rodríguez-Castillo A, Ortiz-Alcántara JM, Gonzalez-Durán E, Segura-Candelas JM, Pérez-Agüeros SI, Escobar-Escamilla N, Méndez-Tenorio A, Diaz-Quiñonez JA, Ramirez-González JE. 2015. Full-genome sequence of a novel varicella-zoster virus clade isolated in Mexico. Genome Announc 3(4):e00752-15. doi:10.1128/genomeA.00752-15. Received 2 June 2015. Accepted 4 June 2015. Published 9 July 2015. Copyright © 2015 Garcés-Ayala et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.