We investigated differences in the five currently-available datasets of column-integrated CO2 concentrations (XCO2) retrieved from spectral soundings collected by Greenhouse gases Observing SATellite (GOSAT), and assessed their impact on regional CO2 flux estimates. We did so by estimating the fluxes from each of the five XCO2 datasets combined with surface-based CO2 data, using a single inversion system. The five XCO2 datasets are available in raw and bias-corrected versions, and we found that the bias-corrections diminish the range of the five coincident values by ~30% on average. The departures of the five individual inversion results (annual-mean regional fluxes based on XCO2-surface combined data) from the surface-data-only results were close to one another in some terrestrial regions where spatial coverage by each XCO2 dataset was similar. The mean of the five annual global land uptakes was 1.7 ± 0.3 GtC yr−1, and they were all smaller than that estimated from the surface-based data alone.