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Elsevier, Ultramicroscopy, 1-4(71), p. 303-309
DOI: 10.1016/s0304-3991(97)00075-2
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Near-field fluorescence microscopy of cells
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Abstract
We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.