Humana Press, Methods in Molecular Biology, p. 173-189, 2012
DOI: 10.1007/978-1-61779-882-5_12
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In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses: Cucumber mosaic virus (CMV), including the CMV Y-Satellite, Turnip mosaic virus (TuMV), Potato leaf roll virus (PLRV), and Tomato spotted wilt virus (TSWV) from inoculated Arabidopsis thaliana plants (Fusaro et al. EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA).