Results of development of spectrophotometric technique for human hair testing in the laboratory and clinical conditions are presented. Hair tester, which can be used for measurements of optical parameters of in vitro hair samples at three fixed wavelengths of illuminating light, is described. Usage of CCD sensor as photodetector allows one to provide spatial-resolved analysis of the hair optical properties and to measure geometric parameters of the hair sample. Experimental results obtained with designed instrumentation and technique are presented.