In this study efficacy of near neutral and alkaline pH electrolyzed oxidizing waters to reduce aerobic plate counts (AC) and Enterobacteriaceae (EC) from uninoculated fresh cattle hides and Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 from inoculated hides were determined. Fresh hides were cut in to 15 by 20 cm pieces and subjected to a total of eight different treatment solutions; near neutral pH EO water (NEW-pH 6.5 at room temperature, 150 mg/L available chlorine), alkaline pH EO water (AEO-pH 11.6 at room temperature), hot alkaline pH EO water at 43 °C (HAEO-pH 11.60), alkaline pH EO water spray followed by 150 mg/L available chlorine containing near neutral pH EO water spray (A-NEW-both at room temperature), Blitz™ (PAA, pH 3.02 at room temperature), 5% lactic acid (LA, pH 2.04 at room temperature), deionized water (W) and no treatment (Control). For each treatment, 60 ml treatment solution was sprayed on hide using a hand held sprayer. Similar treatment protocol was employed to treat hide pieces inoculated with E. coli O157:H7 and S. Typhimurium DT 104. Five percent lactic acid spray treatment was found to be the most effective treatment and achieved 2.77, 2.74, 2.75 and 2.98 log CFU/cm2 of AC, EC, E. coli O157:H7 and S. Typhimurium DT 104 reductions, respectively. All EO water treatments were equally effective in reducing all target microorganisms, except E. coli O157:H7. HAEO and A-NEW treatments yielded significant reduction of E. coli O157:H7 compared to other EO water treatments. These results indicate that various EO water treatments could become viable options to reduce pathogens on hide during slaughter.