The objective of this research was to determine the effects of using tanks with different bottom areas/volumes on the growth and reproduction of the European cuttlefish, Sepia officinalis. One hundred and eighty-four juvenile cuttlefish (46.1 ± 20.9 g) were used to test replicates of 9000 L (6.67 m 2), 750 L (1.54 m 2) and 250 L (0.79 m 2 – control). Growth and reproduction data were registered. In addition, egg proximate composition differences were assessed by collect-ing egg samples from each tank. Regarding growth, no statistical differences between tank types were found (P > 0.05), but total absolute mortality was lower in 9000 L tanks. As for reproduction, differences were found (P < 0.05) between individual tanks for mean egg (MEW), male (MW♂) and hatchling (MHW) weights; and egg batch number. One of the 9000 L tanks displayed the highest overall and individual fecundity (16 593 and 1383 eggs respectively) and egg viability (~72%) ever obtained in our facilities. Differences (P < 0.05) regarding egg proxi-mate composition of different tanks were noted. Significant (P < 0.05) correlations were estab-lished between the duration of reproduction stage and batch, MEW and MW♀, egg viability and MHW, and MHW and egg ash content. The present results might be related to sex ratios, differences in weight between ♀ and ♂, and/or derived from breeders condition.