Two adult free-ranging Eurasian Beavers (Castor fiber) were observed depositing anal gland secretion at the border of thei territory byeverting the "cloaca", protruding the anal gland openings and rubbing them against the surface as the anin1a walked over the scent mound. We suggest that anal gland secretion applied to scent marks on land has some as ye unknown function in territory defense of the Eurasian Beaver.