Elsevier, Biosensors and Bioelectronics, 6(12), p. 499-510
DOI: 10.1016/s0956-5663(97)00007-9
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An amperometric flow injection immunoanalysis (FIIA) system based on an immunoreactor with immobilized biocomponents on a silica surface has been developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). In the antigen coating mode the hapten was immobilized and monoclonal primary antibody against 2,4-D together with alkaline phosphatase (AP)-labelled secondary antibody were used as sensing elements in a titration assay. In the antibody coating mode a biotinylated monoclonal antibody was immobilized on the surface of the immunoreactor and a 2,4-D-AP-conjugate was used for detection. For electrochemical measurements p-aminophenol enzymatically generated from p-aminophenyl phosphate was oxidized at a carbon working electrode at + 150 mV versus Ag/AgCl. The system enabled the determination of 2,4-D in drinking water samples in the range from 0·2 to 70 μg/l. The whole system was computer controlled with a measuring time of 12 min for one determination.