Background: Allergic diseases, including bronchial asthma is considered one of the pandemics of this century. Its prevalence is increasing and in Colombia, the high morbidity and cost are a public health problem. Asthma is a multigenic entity with a complex component that does not follow the laws of classical Mendelian inheritance. The report of the Global Strategy for the Prevention and Control of Asthma adopts the concept that genetic charge is a risk factor in asthma. Current genetic composition of the population of Latin America Including the Colombian Caribbean populations is molded in history by a mix of African, European and Native Americans; a process that takes place in the context of a broad geographical and social stratification. The study of the genetics of asthma in a population like Colombia is complicated by the diversity and heterogeneity of this ethnic group .In this study we aimed to examine whether genetic ancestry was associated with asthma susceptibility in a multiethnic group of individual settled in the Caribbean coast. Methods: This was an analytical case-control study to examine the association between ancestry and asthma. Samples from 50 asthmatic children and 50 healthy control living in Barranquilla, a city localized up to the north in Colombia were Genotype and analyzed. The frequencies of 46 AIM-InDels and the ancestral components were calculated using STRUCTURE v2.3.3 with a burnin length of 100,000 followed by 100,000 MCMC, with a K = 3, considering the ''Admixture Model'' and correlated allele frequencies. The average ancestry of each group and the odds ratio (OR) of each ancestral percentage over the susceptibility of the disease by logistic regression models were estimated; P values <0.05 are significant. Results: European ancestry was the most prevalent in this population study with 47.2 ± 8.7% in asthmatics and 47.1 ± 8.2% in controls, respectively. The Native American component showed a 28.8 ± 7% and 28.3 ± 6.5% respectively and the African ancestral component had the lowest percentage (24% ± 8.4% and 24.6 ± 9.8, respectively). However, none of the ancestral components showed statistically significant difference between the study groups and any of the ancestral components were not associated as a risk factor for asthma susceptibility. Conclusion: Our results show that this Caribbean population has an ancestral tri-ethnic mix. Also our data is not in consonance with the results described in the literature where low American native ancestral and high African ancestry component have been associated with susceptibility to the diseases.