The hydrochemical features of Ob Inlet in the open-water time were characterized using the data of the surveys during two seasons of different water content. The inlet was subdivided into the “riverine,” “marine,” and intermediate areas being different in the factors determining their hydrochemical regime. The processes occurring in each of the distinguished areas were considered in detail. Special attention was paid to the hydrochemical features in the mixing area of the fresh and saline waters (the frontal zone) within the periods of the maximum and minimum biological activity in the aquatic area of Ob Inlet.