SAGE Publications, Journal of Histochemistry & Cytochemistry, 3(35), p. 327-333, 1987
DOI: 10.1177/35.3.3546482
Elsevier, Ultramicroscopy, 2(21), p. 202
DOI: 10.1016/0304-3991(87)90132-x
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We have developed a simple and effective method to embed whole yeast cells in Lowicryl resins with excellent ultrastructural and antigenic preservation. Using affinity-purified antibodies eluted from electrophoretically separated proteins transferred to nitrocellulose, we have shown by immunoelectron microscopy that two glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase and hexokinase, are present in the cytoplasm and the nucleus. Carboxypeptidase Y is localized in the yeast vacuole. These results agree with earlier localization studies based on subcellular fractionation.