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American Society of Parasitologists, The Journal of Parasitology, 3(92), p. 668-670
DOI: 10.1645/ge-706r.1
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Nourseothricin Acetyltransferease: A Positive Selectable Marker for Toxoplasma gondii
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Abstract
Molecular analysis of parasite genomes will require new molecular genetic tools. The nat1 gene of Streptomyces noursei encodes nourseothricin acetyltransferase, conferring resistance to the aminoglycoside antibiotic nourseothricin. Electroporation of nat1 cassettes into RH or Prugniaud strains of Toxoplasma gondii allows for selection of stable nourseothricin-resistant clones.