Published in
Springer, Biotechnology and Bioprocess Engineering, 2(11), p. 164-167, 2006
DOI: 10.1007/bf02931902
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Production of hepatitis B core antigen in a stirred tank bioreactor: The influence of temperature and agitation
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Mung Ing Chua,
Ghee Sung Chua,
Michelle Yeen Tan Ng,
Dayang Radiah Awang Biak,
Wen Siang Tan ,
Tau Chuan Ling
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Abstract
The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.