The aim of the study was to analyse the scent marking patterns in frequency, type and position throughout the reproductive period of two pairs of Canis lupus baileyi housed in two zoos (LZ = Leon and ZZ = Zacango), in terms of newly and established pairs. Focal behaviour sampling was used to register 1,211 scent markings. Data were grouped in three periods: before, during, after and posterior to the reproductive season. Between newly formed pairs no significant difference was found in the previous period (U = 6.50, p>0.065); also, no significant difference was observed in the double marking (U = 54.0, p>0.083) during the reproductive season, although it was greater in the LZ compared to the ZZ pair. After commingling together for one year the established LZ couple, showed an increase in double marking (U = 16.5, p<0.001) during the mating period in comparison with the prior year. Male wolves marked with greater frequency with the leg raised in both, the double and single marking, whereas females marked more in a squatting position. It is concluded that scent marking is different in recently formed pairs in captivity, which are found in a reduced space and have not free choice to elect its mate.