Spectrochimica Acta Part A: Molecular Spectroscopy, 3(50), p. 577-582
DOI: 10.1016/0584-8539(94)80170-3
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A novel method for determining absorbance is described that is especially applicable to remote environmental measurements. Fluorescent dyes (Stilbene 420 and 3-hydroxyflavone) placed before and after the sample provide an internally-referenced signal proportional to transmission that is independent of the source light intensity. Concentrations as low as 50 ppb (w/w) of biphenyl in ethanol were detectable using this technique. This detection limit is comparable to that of standard laboratory absorption spectrometer measurements.