We demonstrate an optical amplification of organic dye within a TiO2 inverse-opal (IO) distributed feedback (DFB) reflector prepared by a slide-coating method. Highly reflective TiO2 IO film was fabricated by slide coating the binary aqueous dispersions of polystyrene microspheres and charge-stabilized TiO2 nanoparticles on a glass slide and subsequently removing the polymer-opal template. TiO2 IO film was infiltrated, in turn, with the solutions of DCM, a fluorescent dye in various solvents with different indices of refraction. Optical pumping by frequency-doubled Nd:YAG laser resulted in amplified spontaneous emission in each dye solution. In accordance with the semi-empirical simulation by the FDTD method, DCM in ethanol showed the best emission/stopband matching for the TiO2 IO film used in this study. Therefore, photo excitation of a DCM/ethanol cavity showed a single-mode DFB lasing at 640 nm wavelength at moderate pump energy.