Ag, Cu metallic and bimetallic nanoparticles (NPs) with diverse compositions were efficiently synthesized using the fruit latex of Achrassapota Linn. Spectroscopic and cyclic voltammetry results suggested that reduction of Ag was assisted by ascorbic acid, reducing sugars and other phenolic compounds present in the latex. However, the reduction of Cu and alloy NPs required additional ascorbic acid. Comparative in vitro toxicity of as synthesized nanoparticle solution was assessed in 3T3L1 cells using MTT assay and fluorescentmicroscopy. A Minimal impact was observed on cell viability and morphology during 72 hours. This demonstrates great potential for use in biomedical applications such as cellular imaging or photothermal therapy.