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Springer Nature [academic journals on nature.com], Journal of Antibiotics, 2(61), p. 89-93, 2008

DOI: 10.1038/ja.2008.116

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Chemical Synthesis and Characterization of J46 Peptide, an Atypical Class IIa Bacteriocin from Lactococcus lactis subsp. cremoris J46 Strain

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Bacteriocin J46 is a 27-residue polypeptide produced by Lactococcus lactis subsp. cremoris J46 in fermented milk. The natural form of J46 (nJ46) exhibits a broad antimicrobial spectrum. Herein, we produced the synthetic form of J46 (sJ46) by solid-phase chemical synthesis. The biochemical and physico-chemical properties of sJ46, as well as its antimicrobial activity, were found to be identical to those of its natural counterpart nJ46. It showed a potent antimicrobial activity against both lactic acid bacteria and other Gram-positive microorganisms. 1H-NMR conformational analysis of sJ46 indicates that it adopts a flexible random coil structure.Keywords: bacteriocin J46, antimicrobial activity, synthetic peptide, conformational analysis, Lactococcus lactis subsp. cremoris J46