The current study focuses on the detection and characterization of potentially pathogenic Aeromonas sobria from fish Silver carp (Hypophthalmichthys molitrix). Assessment of clinical, microbiological, pathological and biochemical characteristics of Aeromonas sobria were taken into account in order to understand the epidemiology, frequency and occurrence of this infection. Clinically the infected fish (Hypophthalmichthys molitrix) was observed for various types of symptoms. A total of 33 colonies of Aeromonas sobria strain were isolated from 20 cultured Hypophthalmichthys molitrix, collected from controlled fish pond. Microscopic examination revealed that the strains were rod-shaped, Gram negative bacteria. The revealed percent probability identification of A. sobria from the biochemical characterization in VITEK system was 93% with gram negative (GN) card. The histopathology of Gills caused by this bacterium, Aeromonas sobria indicate haemorrhagic gill epithelia and epithelial hyperplasia. Lamelar epithelial hypertrophy and hyperplasia with degenerative changes of the epithelium and hypertrophic epitheliocystis infected cells on gills of Hypophthalmichthys molitrix were observed during the present study.