In this study, stable and homogenous thin films of multiwalled carbon nanotubes (MWCNTs) were obtained on conducting surface using ciprofloxacin (CF, fluoroquinolone antibiotic) as an effective-dispersing agent. Further, MWCNTs/CF film modified electrodes (glassy carbon and indium tin oxide-coated glass electrode) are used successfully to study the direct electrochemistry of proteins. Here, cytochrome C (Cyt-C) was used as a model protein for investigation. A MWCNTs/CF film modified electrode was used as a biocompatible material for immobilization of Cyt-C from a neutral buffer solution (pH 7.2) using cyclic voltammetry (CV). Interestingly, Cyt-C retained its native state on the MWCNTs/CF film. The Cyt-C adsorbed MWCNTs/CF film was characterized by scanning electron microscopy (SEM), UV-visible spectrophotometry (UV-vis) and CV. SEM images showed the evidence for the adsorption of Cyt-C on the MWCNTs/CF film, and UV-vis spectrum confirmed that Cyt-C was in its native state on MWCNTs/CF film. Using CV, it was found that the electrochemical signal of Cyt-C was highly stable in the neutral buffer solution and its redox peak potential was pH dependent. The formal potential (-0.27 V) and electron transfer rate constant (13 +/- 1 s(-1)) were calculated for Cyt-C on MWCNTs/CF film modified electrode. A potential application of the Cyt-C/MWCNTs/CF electrode as a biosensor to monitor H(2)O(2) has been investigated. The steady-state current response increases linearly with H(2)O(2) concentration from 2 x 10(-6) to 7.8 x 10(-5) M. The detection limit for determination of H(2)O(2) has been found to be 1.0 x 10(-6) M (S/N = 3). Thus, Cyt-C/MWCNTs/CF film modified electrode can be used as a biosensing material for sensor applications.