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Nature Research, Nature Methods, 1(13), p. 59-62, 2015

DOI: 10.1038/nmeth.3667

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Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

Journal article published in 2015 by Raymond G. Sierra, Cornelius Gati ORCID, Hartawan Laksmono, E. Han Dao, E. Han Dao, Sheraz Gul, Franklin Fuller, Jan Kern, Ruchira Chatterjee, Mohamed Ibrahim, Aaron S. Brewster, Iris D. Young, Tara Michels-Clark, Andrew Aquila ORCID, Mengning Liang and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).