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Wiley, FEBS Letters, 24(580), p. 5690-5696, 2006

DOI: 10.1016/j.febslet.2006.09.020

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Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria

Journal article published in 2006 by Anna Maria Sardanelli, Anna Signorile, Rosanna Nuzzi, Domenico De Rasmo, Domenico De Rasmo ORCID, Zuzana Technikova-Dobrova, Zdenek Drahota, Antonella Occhiello, Alessandra Pica, Sergio Papa
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Abstract

Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by (32)P-phosphorylated C-PKA and of AKAP by (32)P-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody.