Phytochemical investigation of the whole Morettia philaena (Del.) DC. plant led to the identification of nine flavonoids isolated using chromatographic techniques. These are kaempferol, kaempferol 3-O-β-glucopyranoside, kaempferol3, 7di-O-β-glucopyranoside,kaempferol3-O-β-sophoroside-7-O-β-glucopyranoside, quercetin, quercetin 3-O-β-glucopyranoside, quercetin 3-O-β-gentobioside, orientin and isoorientin. Their structures were established through chemical and spectral analysis. The antimicrobial activity of the isolated flavonoids and the aqueous methanol extract against six bacteria and one fungus species was studied. Among them, the isolated aglycones, mono-O- glycosides and C- glycosides exhibited interesting high activity against most of the tested organisms.