The round reduction on block cipher is a fault injection attack in which an attacker inserts temporary errors in cryptographic devices and extracts a secret key by reducing the number of operational round. In this paper, we proposed an improved round reduction method to retrieve master keys by injecting a fault during operation of loop statement in the Triple DES. Using laser fault injection experiment, we also verified that the proposed attack could be applied to a pure microprocessor ATmega 128 chip in which the Triple DES algorithm was implemented. Compared with previous attack method which is required 9 faulty-correct cipher text pairs and some exhaustive searches, the proposed one could extract three 56-bit secret keys with just 5 faulty cipher texts.