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Taylor and Francis Group, Biotechnic & Histochemistry, 2(85), p. 127-131, 2010

DOI: 10.3109/10520290903149604

Taylor and Francis Group, Biotechnic & Histochemistry, p. 1-5

DOI: 10.1080/10520290903149604

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Three-color intranuclear staining for measuring mitosis and apoptosis in cells transfected with a GFP-tagged histone

Journal article published in 2009 by J. Rios Doria, J. Meléndez, An N. Monteiro ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Green fluorescent protein (GFP) has become a powerful tool for monitoring the expression of transfected genes by flow cytometry including GFP-tagged histones for tracking chromatin and elucidating histone function. We describe here a method for simultaneous detection of three nucleus-localized signals: a GFP-tagged histone, DNA content and detection of phosphorylated histone H3, which labels mitotic cells. We also demonstrate another application of this method for simultaneous detection of a GFP-tagged histone, DNA content, and cleaved caspase-3.