Fe single crystal film with thickness of 45 monolayer was fabricated on vicinal Si(111) substrate using ultrathin p(2×2) iron silicide as buffer layer. Scanning tunneling microscope images show that the Fe nanoclusters form chains on vicinal substrate. The first- and second-order magnetocrystalline anisotropies, uniaxial magnetic anisotropy constants of the films were obtained by fitting the ferromagnetic resonance data. The sixfold symmetry of the in-plane resonance field for Fe(111) film was changed into the superposition of a fourfold and a twofold contribution due to the effect of the vicinal substrate.