American Society for Microbiology, Applied and Environmental Microbiology, 3(77), p. 1097-1100, 2011
DOI: 10.1128/aem.01541-10
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ABSTRACT We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli . This, in turn, allows the isolation of highly pure FMN.