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Sociedade Brasileira de Química, SBQ, Journal of the Brazilian Chemical Society, 6a(16), p. 1226-1232, 2005

DOI: 10.1590/s0103-50532005000700021

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An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process

Journal article published in 2005 by Eduardo P. Borges, E. P. Borgesa, Boaventura F. Reis ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In this work an automatic flow procedure for L-alanine determination in the synthesis medium without a prior sample treatment is described. The method was based on oxidation of L-alanine by L-amino acid oxidize followed by the reaction of the H2O2 with luminol and chemiluminescence detection. In order to overcome the severe alkaline condition of the synthesis medium, a zone sampling strategy was employed for automatic sample dilution and pH adjustment. The procedure allows L-alanine determination in the range of 0.5 to 25.0 mmol L-1, a limit of detection of 0.08 mmol L-1 and a variation coefficient lower than 4% (n = 10). Other advantageous feature such as a sampling throughput of 44 determination per hour and low reagent consumption, 0.2 mg luminol and 16.4 mg hexacinoferrate(III) per determination, respectively, were also achieved.