The bioartificial liver is based on the liver function of the hepatocytes cultured in a bioreactor for a temporary effective therapy for treating a chronic liver damage. This paper presents the evaluation of a hybrid cellular scaffold that has a polymer part and a biological part, whose surface has been modified with polypyrrole plasma and seeded with non-parenchymal liver cells, cell cultures for efficient three-dimensional hepatocyte reported in radial flow bioreactor. Bioreactor radial flow was used at a flow rate of 7 ml/min with steel parts of food grade and glass were cultured hepatocytes of the HepG2 cell line on polypyrrole iodine coated Poly (L-lactic acid) scaffolds and endothelial cells, these results were compared with data of crops with HepG2 cells and only with uncoated scaffolds. Cell proliferation was increased by 30%. These results indicate that the PLLA/Ppy-I with HUVEC cells scaffold may be a promising development of culture methods for the functional application for potential future pharmacological and clinical use in bioartificial livers.